◦ The working principle of the Spectrophotometer is based on Beer-Lambert's law which states that the amount of light absorbed by a color solution is directly proportional to the concentration of the solution and the length of a light path through the solution. That is why the natural baseline of the instrument is not flat. If you do a zero, it is done at only the starting wavelength. Make sure each one is lean (if they aren't, clean. Before using the Spectronic 200, follow these steps to make sure that no damage or injury will occur due to faulty or careless behavior. If the step size is not set for you, you will have to set the step size.